Applications

Selected publications

Read scientific data on applications of the Piuma and Chiaro nanoindentation systems. For more information on applications also read the first blog!

Native tissues:
Xie, Meng, Pavel Gol’din, Anna Nele Herdina, Jordi Estefa, Ekaterina V. Medvedeva, Lei Li, Phillip T. Newton, et al. “Evolution of the Growth Plate into a Spatially Separated Structure Allows Bone Growth on Land.BioRxiv, September 8, 2019, 571612.

Tarafder, Solaiman, Joseph Gulko, Daniel Kim, Kun Hee Sim, Shawn Gutman, Jian Yang, James L. Cook, and Chang H. Lee. “Effect of Dose and Release Rate of CTGF and TGFβ3 on Avascular Meniscus Healing.Journal of Orthopaedic Research 37, no. 7 (2019): 1555–62.

Zhang, Xianzhu, Dandan Cai, Feifei Zhou, Jie Yu, Xinyu Wu, Dongsheng Yu, Yiwei Zou, et al. 2019. “Targeting Downstream Subcellular YAP Activity as a Function of Matrix Stiffness with Verteporfin-Encapsulated Chitosan Microsphere Attenuates Osteoarthritis.Biomaterials, December, 119724. 

Tarafder, S., Gulko, J., Sim, K. H., Yang, J., Cook, J. L., & Lee, C. H. (2018). Engineered Healing of Avascular Meniscus Tears by Stem Cell Recruitment. Scientific Reports8(1). 

Bos, Ernst Jan, Mieke Pluemeekers, Marco Helder, Nikolay Kuzmin, Koen van der Laan, Marie-Louise Groot, Gerjo van Osch, and Paul van Zuijlen. 2018. “Structural and Mechanical Comparison of Human Ear, Alar, and Septal Cartilage.Plast Reconstr Surg Glob Open 6 (1).

Vindas Bolaños, R. A., S. M. Cokelaere, J. M. Estrada McDermott, K. E. M. Benders, U. Gbureck, S. G. M. Plomp, H. Weinans, J. Groll, P. R. van Weeren, and J. Malda. 2017. “The Use of a Cartilage Decellularized Matrix Scaffold for the Repair of Osteochondral Defects: The Importance of Long-Term Studies in a Large Animal Model.Osteoarthritis and Cartilage, Negative findings in in vivo models of osteoarthritis (OA), 25 (3): 413–420.

Moshtagh, P. R., Pouran, B., Korthagen, N. M., Zadpoor, A. A., & Weinans, H. (2016). Guidelines for an optimized indentation protocol for measurement of cartilage stiffness: The effects of spatial variation and indentation parametersJournal of Biomechanics49(14), 3602–3607. 

Contractile force measurements:
Acun, Aylin, Trung Dung Nguyen, and Pinar Zorlutuna. “In Vitro Aged, HiPSC-Origin Engineered Heart Tissue Models with Age-Dependent Functional Deterioration to Study Myocardial Infarction.Acta Biomaterialia 94 (August 1, 2019): 372–91. 

Nguyen, D. T., Nagarajan, N., & Zorlutuna, P. (2018). Effect of Substrate Stiffness on Mechanical Coupling and Force Propagation at the Infarct Boundary. Biophysical Journal, 115(10), 1966–1980.

Acun, A., Nguyen, T. D., & Zorlutuna, P. (ter perse). An aged human heart tissue model showing age-related molecular and functional deterioration resembling the native heart.

Cardiac cells:
Emig, Ramona, Callum M. Zgierski-Johnston, Friedhelm Beyersdorf, Bartosz Rylski, Ursula Ravens, Wilfried Weber, Peter Kohl, Maximilian Hörner, and Rémi Peyronnet. “Human Atrial Fibroblast Adaptation to Heterogeneities in Substrate Stiffness.Frontiers in Physiology 10 (2020).

Caporizzo, Matthew Alexander, Christina Yingxian Chen, Alexander Koizumi Salomon, Kenneth B. Margulies, and Benjamin L. Prosser. “Microtubules Provide a Viscoelastic Resistance to Myocyte Motion.Biophysical Journal 115, no. 9 (November 6, 2018): 1796–1807.

Xie, S., Zhang, T., Wang, J., Zhao, F., Zhang, Y., Yao, W., … Zhou, J. (2018). Matrix stiffness determines the phenotype of vascular smooth muscle cell in vitro and in vivo : Role of DNA methyltransferase 1Biomaterials155, 203–216.

Chen, C. Y., Caporizzo, M. A., Bedi, K., Vite, A., Bogush, A. L., Robison, P., … Prosser, B. L. (2018). Suppression of detyrosinated microtubules improves cardiomyocyte function in human heart failure. Nature Medicine24(8), 1225–1233.

Cardiac tissue:
Meekel, Jorn P., Giorgio Mattei, Victor S. Costache, Ron Balm, Jan D. Blankensteijn, and Kak K. Yeung. “A Multilayer Micromechanical Elastic Modulus Measuring Method in Ex Vivo Human Aneurysmal Abdominal Aortas.Acta Biomaterialia 96 (September 15, 2019): 345–53.

Engineered vascular tissue:
Arias, Sandra L., Akshath Shetty, Joshua Devorkin, and Jean-Paul Allain. “Magnetic Targeting of Smooth Muscle Cells in Vitro Using a Magnetic Bacterial Cellulose to Improve Cell Retention in Tissue-Engineering Vascular Grafts.Acta Biomaterialia 77 (September 1, 2018): 172–81.

er, Oren, Nivi Hirsch‐Attas, Miriam Schlossberg, Fani Brofman, Roy Friedman, Meital Kupervaser, Danny Kitsberg, and Amnon Buxboim. “A Unified Linear Viscoelastic Model of the Cell Nucleus Defines the Mechanical Contributions of Lamins and Chromatin.” Advanced Science (2020): 1901222.

Karoutas, Adam, Witold Szymanski, Tobias Rausch, Sukanya Guhathakurta, Eva A. Rog-Zielinska, Remi Peyronnet, Janine Seyfferth, et al. “The NSL Complex Maintains Nuclear Architecture Stability via Lamin A/C Acetylation.Nature Cell Biology 21, no. 10 (October 2019): 1248–60.

Nguyen, D. T., Nagarajan, N., & Zorlutuna, P. (2018). Effect of Substrate Stiffness on Mechanical Coupling and Force Propagation at the Infarct BoundaryBiophysical Journal115(10), 1966–1980.

Xie, S.-A., Zhang, T., Wang, J., Zhao, F., Zhang, Y.-P., Yao, W.-J., … Zhou, J. (2018). Matrix stiffness determines the phenotype of vascular smooth muscle cell in vitro and in vivo : Role of DNA methyltransferase 1Biomaterials155, 203–216.

Stem cells:

Wangler, S., U. Menzel, Z. Li, J. Ma, S. Hoppe, L. M. Benneker, M. Alini, S. Grad, and M. Peroglio. 2019. “CD146/MCAM Distinguishes Stem Cell Subpopulations with Distinct Migration and Regenerative Potential in Degenerative Intervertebral Discs. Osteoarthritis and Cartilage 27 (7): 1094–1105. 

Cells on gels:
Nichol, Robert H., Timothy S. Catlett, Massimo M. Onesto, Drew Hollender, and Timothy M. Gómez. “Environmental Elasticity Regulates Cell-Type Specific RHOA Signaling and Neuritogenesis of Human Neurons.Stem Cell Reports, November 7, 2019.

Yue, X., Nguyen, T., Zellmer, V., Zhang, S., & Zorlutuna, P. (2018). Stromal cell-laden 3D hydrogel microwell arrays as tumor microenvironment model for studying stiffness dependent stromal cell-cancer interactionsBiomaterials170, 37–48.

Li, Minghao, and Jinhye Bae. “Tunable Swelling and Deswelling of Temperature- and Light-Responsive Graphene Oxide-Poly(N-Isopropylacrylamide) Composite Hydrogels.Polymer Chemistry, March 4, 2020.
Pouraghaei, Sevda, Fathollah Moztarzadeh, Chider Chen, Sahar Ansari, and Alireza Moshaverinia. “Microenvironment Can Induce Development of Auditory Progenitor Cells from Human Gingival Mesenchymal Stem Cells.ACS Biomaterials Science & Engineering, March 10, 2020.
Mattei, Giorgio, Ludovica Cacopardo, and Arti Ahluwalia. “Engineering Gels with Time-Evolving Viscoelasticity.Materials 13, no. 2 (January 2020): 438.

Giobbe, Giovanni Giuseppe, Claire Crowley, Camilla Luni, Sara Campinoti, Moustafa Khedr, Kai Kretzschmar, Martina Maria De Santis, et al. “Extracellular Matrix Hydrogel Derived from Decellularized Tissues Enables Endodermal Organoid Culture.Nature Communications 10, no. 1 (December 11, 2019): 1–14. 

Yue, Liang, Shan Wang, Verena Wulf, and Itamar Willner. “Stiffness-Switchable DNA-Based Constitutional Dynamic Network Hydrogels for Self-Healing and Matrix-Guided Controlled Chemical Processes.Nature Communications 10, no. 1 (October 21, 2019): 1–10.

Ye, Dongdong, Chunyu Chang, and Lina Zhang. “High-Strength and Tough Cellulose Hydrogels Chemically Dual Cross-Linked by Using Low- and High-Molecular-Weight Cross-Linkers.Biomacromolecules 20, no. 5 (May 13, 2019): 1989–95.

Arias, Sandra L., Akshath Shetty, Joshua Devorkin, and Jean-Paul Allain. “Magnetic Targeting of Smooth Muscle Cells in Vitro Using a Magnetic Bacterial Cellulose to Improve Cell Retention in Tissue-Engineering Vascular Grafts.Acta Biomaterialia 77 (September 1, 2018): 172–81.
Reynolds, Daniel S., Kristen M. Bougher, Justin H. Letendre, Stephen F. Fitzgerald, Undina O. Gisladottir, Mark W. Grinstaff, and Muhammad H. Zaman. “Mechanical Confinement via a PEG/Collagen Interpenetrating Network Inhibits Behavior Characteristic of Malignant Cells in the Triple Negative Breast Cancer Cell Line MDA.MB.231.Acta Biomaterialia 77 (September 1, 2018): 85–95.

Mattei, G., Cacopardo, L., & Ahluwalia, A. (2017). Micro-Mechanical Viscoelastic Properties of Crosslinked Hydrogels Using the Nano-Epsilon Dot MethodMaterials10(8), 889.

Sarker, B., Singh, R., Zehnder, T., Forgber, T., Alexiou, C., Cicha, I., … Boccaccini, A. R. (2016). Macromolecular interactions in alginate–gelatin hydrogels regulate the behavior of human fibroblastsJournal of Bioactive and Compatible Polymers32(3), 309–324.

Acun, A., Nguyen, T. D., & Zorlutuna, P. (ter perse). An aged human heart tissue model showing age-related molecular and functional deterioration resembling the native heart.

Gels:
Zhang, Haihui, Feng Lin, Jianyong Huang, and Chunyang Xiong. “Anisotropic Stiffness Gradient-Regulated Mechanical Guidance Drives Directional Migration of Cancer Cells.Acta Biomaterialia, February 8, 2020.

Deckx, Sophie, Daniel M. Johnson, Marieke Rienks, Paolo Carai, Elza Van Deel, Jolanda Van der Velden, Karin R. Sipido, Stephane Heymans, and Anna-Pia Papageorgiou. 2019. “Extracellular SPARC Increases Cardiomyocyte Contraction during Health and Disease.PloS One 14 (4): e0209534. 

Brauer, Erik, Evi Lippens, Oliver Klein, Grit Nebrich, Sophie Schreivogel, Gabriela Korus, Georg N. Duda, and Ansgar Petersen. “Collagen Fibrils Mechanically Contribute to Tissue Contraction in an In Vitro Wound Healing Scenario.Advanced Science 6, no. 9 (March 14, 2019).
Diba, Mani, Alessandro Polini, Daniela Geta Petre, Yang Zhang, and Sander C. G. Leeuwenburgh. “Fiber-Reinforced Colloidal Gels as Injectable and Moldable Biomaterials for Regenerative Medicine.Materials Science and Engineering: C 92 (November 1, 2018): 143–50.
Friedrich, O., D. Schneidereit, Y. A. Nikolaev, V. Nikolova-Krstevski, S. Schürmann, A. Wirth-Hücking, A. L. Merten, D. Fatkin, and B. Martinac. 2017. “Adding Dimension to Cellular Mechanotransduction: Advances in Biomedical Engineering of Multiaxial Cell-Stretch Systems and Their Application to Cardiovascular Biomechanics and Mechano-Signaling.Progress in Biophysics and Molecular Biology, Cardiac Mechanics and Electrics: it takes two to tango, 130 (November): 170–191. 
Deel, Elza D. van, Aref Najafi, Dulce Fontoura, Erik Valent, Max Goebel, Kim Kardux, Inês Falcão‐Pires, and Jolanda van der Velden. 2017. “In Vitro Model to Study the Effects of Matrix Stiffening on Ca2+ Handling and Myofilament Function in Isolated Adult Rat Cardiomyocytes.The Journal of Physiology 595 (14): 4597–4610.
Coatings: