Find more information here on the various nanoindentation applications of the Optics11 Life instruments
Highlighted Nanoindentation Applications
Development of label-free biophysical marker in osteogenic maturation: Massimo Vassali’s group from Genova demonstrated correlation between morpho-mechanical features of osteoblasts and their maturation.
The NSL complex maintains nuclear architecture stability via lamin A/C acetylation: Remi Peyronnet’s group from the UHZ in Freiburg measured the stiffness of the cytoplasm and nuclei with the CHIARO in order to understand nuclear mechanostability.
A multilayer micromechanical elastic modulus measuring method in ex vivo human aneurysmal abdominal aortas: the researchers from VU Medical Center Amsterdam have correlated results of quantitative immunofluorescence and elastic properties of abdominal aortic aneurysm tissue.
Changes in Epithelial and Stromal Corneal Stiffness Occur with Age and Obesity: the group of prof. Vickery Trinkaus-Randall from Boston University School of Medicine has found that elastic modulus was decreased in pre-Type 2 diabetic obese mice while it increased with age.
Environmental elasticity regulates cell-type specific RHOA signaling and neuritogenesis of human neurons: Timothy Gomez et al. used the PIUMA to investigate effects of various stiffness polyacrylamide and collagen hydrogels as an micro-environment for the developing hMNs and hFB neurons.
Stiffness of hydrogels for self-healing & controlled drug delivery matrices: Itamar Willner’s group from Hebrew University utilized the PIUMA to study how stiffness of constitutional dynamic network (CDN)-guided hydrogels can be controlled.
Tailoring the collagen film structural properties via direct laser crosslinking of star-shaped polylactide for robust scaffold formation: Peter Timashev’s group from the RAS in Moscow used the PIUMA to characterize changes in collagen-based scaffolds due to laser-induced curing.
Catechol-mediated and copper-incorporated multilayer coating: An endothelium-mimetic approach for blood-contacting devices: the researchers at Sichuan University, Chengdu, China have included mechanical tests by Piuma to compare coatings before and after copper coordination.
Wintner, Oren, Nivi Hirsch‐Attas, Miriam Schlossberg, Fani Brofman, Roy Friedman, Meital Kupervaser, Danny Kitsberg, and Amnon Buxboim. “A Unified Linear Viscoelastic Model of the Cell Nucleus Defines the Mechanical Contributions of Lamins and Chromatin.” Advanced Science (2020): 1901222.
Karoutas, Adam, Witold Szymanski, Tobias Rausch, Sukanya Guhathakurta, Eva A. Rog-Zielinska, Remi Peyronnet, Janine Seyfferth, et al. “The NSL Complex Maintains Nuclear Architecture Stability via Lamin A/C Acetylation.” Nature Cell Biology 21, no. 10 (October 2019): 1248–60.
Bartolozzi, Alice, Federica Viti, Silvia De Stefano, Francesca Sbrana, Loredana Petecchia, Paola Gavazzo, and Massimo Vassalli. “Development of Label-Free Biophysical Markers in Osteogenic Maturation.” Journal of the Mechanical Behavior of Biomedical Materials 103 (March 1, 2020): 103581.
Hodgkinson, T., Tsimbouri, P. M., Llopis-Hernandez, V., Campsie, P., Scurr, D., Childs, P. G., Phillips, D., Donnelly, S., Wells, J. A., O’Brien, F. J., Salmeron-Sanchez, M., Burgess, K., Alexander, M., Vassalli, M., Oreffo, R. O. C., Reid, S., France, D. J., & Dalby, M. J. (2021). The use of nanovibration to discover specific and potent bioactive metabolites that stimulate osteogenic differentiation in mesenchymal stem cells. Science Advances, 7(9), eabb7921.
Wangler, S., U. Menzel, Z. Li, J. Ma, S. Hoppe, L. M. Benneker, M. Alini, S. Grad, and M. Peroglio. 2019. “CD146/MCAM Distinguishes Stem Cell Subpopulations with Distinct Migration and Regenerative Potential in Degenerative Intervertebral Discs. Osteoarthritis and Cartilage 27 (7): 1094–1105.
Künzel, S. R., Rausch, J. S. E., Schäffer, C., Hoffmann, M., Künzel, K., Klapproth, E., Kant, T., Herzog, N., Küpper, J.-H., Lorenz, K., Dudek, S., Emig, R., Ravens, U., Rog‐Zielinska, E. A., Peyronnet, R., & El‐Armouche, A. (2020). Modeling atrial fibrosis in vitro—Generation and characterization of a novel human atrial fibroblast cell line. FEBS Open Bio, 10(7), 1210–1218.
Coleman, A. K., Joca, H. C., Shi, G., Lederer, W. J., & Ward, C. W. (2020). Tubulin acetylation increases cytoskeletal stiffness to regulate mechanotransduction in striated muscle. BioRxiv, 2020.06.10.144931.
Hoffmann, M., Kant, T. A., Emig, R., Rausch, J. S. E., Newe, M., Schubert, M., Künzel, K., Winter, L., Klapproth, E., Peyronnet, R., Ravens, U., El-Armouche, A., & Künzel, S. R. (2020). Repurposing mesalazine against cardiac fibrosis in vitro. Naunyn-Schmiedeberg’s Archives of Pharmacology.
Chen, C. Y., Caporizzo, M. A., Bedi, K., Vite, A., Bogush, A. L., Robison, P., … Prosser, B. L. (2018). Suppression of detyrosinated microtubules improves cardiomyocyte function in human heart failure. Nature Medicine, 24(8), 1225–1233.
Xie, S.-A., Zhang, T., Wang, J., Zhao, F., Zhang, Y.-P., Yao, W.-J., … Zhou, J. (2018). Matrix stiffness determines the phenotype of vascular smooth muscle cell in vitro and in vivo : Role of DNA methyltransferase 1. Biomaterials, 155, 203–216.
Want to learn more about the various nanoindentation applications of our Nanoindenters? Check out the Application Notes to learn more about the Optics11 Life instruments.