NANOINDENTATION APPLICATIONS
Find more information here on the various nanoindentation applications of the Optics11 Life instruments
Highlighted Nanoindentation Applications
Cells
Development of label-free biophysical marker in osteogenic maturation: Massimo Vassali’s group from Genova demonstrated correlation between morpho-mechanical features of osteoblasts and their maturation.
The NSL complex maintains nuclear architecture stability via lamin A/C acetylation: Remi Peyronnet’s group from the UHZ in Freiburg measured the stiffness of the cytoplasm and nuclei with the CHIARO in order to understand nuclear mechanostability.
Tissue
A multilayer micromechanical elastic modulus measuring method in ex vivo human aneurysmal abdominal aortas: the researchers from VU Medical Center Amsterdam have correlated results of quantitative immunofluorescence and elastic properties of abdominal aortic aneurysm tissue.
Changes in Epithelial and Stromal Corneal Stiffness Occur with Age and Obesity: the group of prof. Vickery Trinkaus-Randall from Boston University School of Medicine has found that elastic modulus was decreased in pre-Type 2 diabetic obese mice while it increased with age.
Hydrogels/Gels
Environmental elasticity regulates cell-type specific RHOA signaling and neuritogenesis of human neurons: Timothy Gomez et al. used the PIUMA to investigate effects of various stiffness polyacrylamide and collagen hydrogels as an micro-environment for the developing hMNs and hFB neurons.
Stiffness of hydrogels for self-healing & controlled drug delivery matrices: Itamar Willner’s group from Hebrew University utilized the PIUMA to study how stiffness of constitutional dynamic network (CDN)-guided hydrogels can be controlled.
Coatings/Biofilms
Tailoring the collagen film structural properties via direct laser crosslinking of star-shaped polylactide for robust scaffold formation: Peter Timashev’s group from the RAS in Moscow used the PIUMA to characterize changes in collagen-based scaffolds due to laser-induced curing.
Catechol-mediated and copper-incorporated multilayer coating: An endothelium-mimetic approach for blood-contacting devices: the researchers at Sichuan University, Chengdu, China have included mechanical tests by Piuma to compare coatings before and after copper coordination.
SELECTED NANOINDENTATION PUBLICATIONS
Read scientific data on applications of the Piuma and Chiaro nanoindentation systems.
For more information on applications also read the first blog!
Stem cells:
Neuronal:
Cornea:
Zhang, Xianzhu, Dandan Cai, Feifei Zhou, Jie Yu, Xinyu Wu, Dongsheng Yu, Yiwei Zou, et al. 2019. “Targeting Downstream Subcellular YAP Activity as a Function of Matrix Stiffness with Verteporfin-Encapsulated Chitosan Microsphere Attenuates Osteoarthritis.” Biomaterials, December, 119724.
Contractile force measurements:
Acun, Aylin, Trung Dung Nguyen, and Pinar Zorlutuna. “In Vitro Aged, HiPSC-Origin Engineered Heart Tissue Models with Age-Dependent Functional Deterioration to Study Myocardial Infarction.” Acta Biomaterialia 94 (August 1, 2019): 372–91.
Hydrogels with cells:
Hydrogels:
Application Notes
Want to learn more about the various nanoindentation applications of our Nanoindenters? Check out the Application Notes to learn more about the Optics11 Life instruments.