In this webinar, you will see how real-time, multi-channel contractility readouts can improve functional assessment of hiPSC-derived skeletal muscle tissues. The speakers share the journey from 2D culture limitations and video-based force estimation to a 3D tissue engineered skeletal muscle model combined with optical fiber sensing. You will learn how continuous measurements inside the incubator enable higher sensitivity, less operator variability, and time-resolved drug response data, including acute and longer-term effects.
Topics Covered:
- Why 2D skeletal muscle cultures fall short for contractility studies, including detachment during stimulation and short culture windows
- How 3D tissue engineered skeletal muscle bundles are fabricated, matured, and electrically stimulated
- Where video-based force readouts hit practical limits, including sensitivity, manual processing, and incubator incompatibility
- How optical fiber sensing enables continuous, real-time contractility monitoring with high sensitivity
- How Cuore supports multi-channel measurements, with multiple tissues monitored simultaneously and automated scanning across a 24-well format
- How to optimize electrical stimulation parameters to reach robust tetanic contractions while avoiding tissue damage
- Examples of pharmacology readouts in real time, including dose-dependent responses, washout effects, and line-to-line variability across hiPSC lines
- How contractility can function as a screening biomarker when molecular markers alone do not predict 3D performance
Who Should Attend?
This webinar is ideal for biomedical professionals, researchers, students, and anyone interested in 3D tissue engineered skeletal muscles (3D-TSEMs), regenerative medicine, tissue development & characterization.
